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方泽民

来源:生命科学学院  发布时间:2017-02-15 访问次数:11672

  • 姓名:方泽民

    邮箱:zemin_fang@163.com

    职称:教授,博士生导师

    联系电话:0551-63861063

    地址:合肥市经开区九龙路111号安徽大学理工C楼209

    邮政编码:230601

      主要研究兴趣包括新颖微生物及酶蛋白的挖掘、改造及应用、微生物与环境互作的调控机制等;近年来主持国家自然科学基金项目等10余项;相关成果在国际主流期刊如Biotech Biofule, Bioresour Technol等发表SCI论文40余篇;申报中国发明专利16项,获得授权11项;是中国微生物学会普通微生物专业委员会青年委员,安徽省生物工程学会理事,安徽省学术技术带头人后备人选;获得安徽省自然科学奖二等奖(2012, 2018),安徽省生物工程学会“优秀青年学者奖”特等奖(2016),长三角生物科技论坛“优秀青年科技工作者”(2017);是Frontiers in Fungal Biology、生物学杂志等杂志编委,Appl Microbiol Biotechnol等杂志审稿人。

    主持项目:

    1. 科技基础资源调查专项子课题,“滩涂区域特色细菌及功能酶资源调查”,2020-2023

    2. 安徽省自然科学基金“杰出青年基金”,“基于“KnowVolution”的漆酶蛋白定向设计”,2020-2023

    3. 国家自然科学基金,“球托霉菌w5菌株蔗糖转运蛋白在真菌植物互作中的功能研究”,2019-2022

    4. 安徽省科技重大专项,“白酒糟转化生产高蛋白饲料及应用”,2018-2019

    5. 安徽省教育厅自然科学研究重大项目,“Hsp70家族成员ThHspA1功能研究”,2017-2019

    6. 国家自然科学基金,“共培养条件下灰盖鬼伞菌漆酶Lcc9表达的转录调控机制”,2014-2016

    7. 国家863计划子课题,“远海大型生物全新天然产物的发现及药物先导化合物的优化”,2013-2015

    8. 教育部高等学校博士学科点专项科研基金联合资助课题(新教师类),“真菌相互作用过程中灰盖鬼伞菌Lcc9功能研究”,2014-2016

    9. 安徽省自然科学基金,“真菌相互作用诱导漆酶表达核心转录因子研究”,2013-2015

    10. 安徽省教育厅自然科学基金重点,“乙醇耐受的新型真菌漆酶筛选”,2013-2015

    11. 安徽大学物质科学与信息技术研究院学科建设开放基金,“漆酶Lcc9的定向进化及在纺织整染中的应用”,2019-2021

    近5年发表文章:

    1. Liu SL, Liu H, Shen C, Fang W, Xiao YZ*, Fang ZM*. Comparison of performances of different fungal laccases in delignification and detoxification of alkali-pretreated corncob for bioethanol production. J Indl Microbiol Biotechnol, 2021, 48, kuab013

    2. Liu JJ#, Cheng J#, Huang M, Shen C, Xu K, Xiao YZ, Pan WJ*, Fang ZM*. Identification of an invertase with high specific activity for raffinose hydrolysis and its application in soymilk treatment. Front. Microbiol. 2021, 12:646801.

    3. Wang XJ, Fang JN, Liu P, Liu JJ, Fang W, Fang ZM*, Xiao YZ. Mucoromycotina fungi possess the ability to utilize plant sucrose as a carbon source: Evidence from Gongronella sp. w5. Front. Microbiol. 2021, 11:591697.

    4. Deng PJ, Meng CY, Wu Y, Xu J, Tang XQ, Zhang XC, Xiao YZ, Wang XT, Fang ZM*, Fang W. An unusual GH1 β-glucosidase from marine sediment with β-galactosidase and transglycosidation activities for superior galacto-oligosaccharide synthesis. Appl Microbiol Biotechnol, 2020 104:4927–4943.

    5. Zhou G, Peng C, Liu XS, Chang F, Xiao YZ, Liu JJ, Fang ZM*. Identification and immobilization of an invertase with high specific activity and sucrose tolerance ability of Gongronella sp. w5 for high fructose syrup preparation. Front. Microbiol. 2020, 11:633.

    6. Yin Q, Zhou G, Peng C, Zhang YL, Kuees U, Liu JJ, Xiao YZ*, Fang ZM*. The first fungal laccase with an alkaline Ph optimum obtained by directed evolution and its application in indigo dye decolorization. AMB Expr, 2019, 9:151

    7. Hu MM, Zhai YW, Zhang YF, Han XY, Fang W, Fang ZM*, Xiao YZ.Marinomonas flavescens sp. nov., isolated from seawater adjacent to Fildes Peninsula, Antarctica. Int J Syst Evol Microbiol, 2019, DOI 10.1099/ijsem.0.003631

    8. Xu GF, Wu YW, Zhang YL, Fang W, Xiao YZ,Fang ZM*. Role of N-glycosylation on the specific activity of a Coprinopsis cinerea laccase Lcc9 expressed in Pichia pastoris. J Biosci Bioeng, 2019, 128(5), 518-524.

    9. Fang W, Xue SS, Deng PJ, Zhang XC, Wang XT, Xiao YZ*, Fang ZM*. AmyZ1: a novel α amylase from marine bacterium Pontibacillus sp. ZY with high activity toward raw starches. Biotechnol Biofuels, 2019, 12:95

    10. Han XY, Zhang J, Liu JJ, Fang W, Fang ZM*, Xiao YZ*. Amphritea opalescens sp. nov., isolated from sediment adjacent to Fildes Peninsula, Antarctica. Int J Syst Evol Microbiol, 2019, DOI 10.1099/ijsem.0.003359

    11.  Hu J, Zhang YL, Xu Y, Sun QY, Liu JJ, Fang W, Xiao YZ, Kües U*, Fang ZM*. Gongronella sp. w5 elevates Coprinopsis cinerea laccase production by carbon source syntrophism and secondary metabolite induction. Appl Microbiol Biotechnol, 2019, 103: 411-425.

    12. Xu GF, Wang JJ, Yin Q, Fang W, Xiao YZ, Fang ZM*. Expression of a thermo- and alkali-philic fungal laccase in Pichia pastoris and its application. Protein Expression and Purification, 2019, 154:16–24

    13. Dong YQ, Sun QY, Zhang YL, Wang XJ, Liu P, Xiao YZ, Fang ZM*. Complete genome of Gongronella sp. w5 provides insight into its relationship with plant. J Biotechnol, 2018, 286:1–4

    14. Chang F, Xue S, Xie X, Fang W, Fang ZM*, Xiao Y. Carbohydrate-binding module assisted purification and immobilization of β-glucosidase onto cellulose and application in hydrolysis of soybean isoflavone glycosides. J Biosci Bioeng, 2018, 125: 185-191.

    15. Chang F, Zhang XB, Pan Y, Lu YX, Fang W, Fang ZM*, Xiao YZ*. Light induced expression of β-glucosidase in Escherichia coli with autolysis of cell. BMC Biotechnol, 2017, 17:74 DOI 10.1186/s12896-017-0402-1

    16. Wang J, Chen Y, Dong Y, Fang W, Fang ZM*, Xiao Y. A simple and efficient method for successful gene silencing of HspA1 in Trametes hirsuta AH28-2. Antonie van Leeuwenhoek, 2017,110:1527–1535

    17.  Zhang Y, Xu Y, Fang W, Wang X, Fang ZM*, Xiao Y. Pseudoruegeria marinistellae sp. nov., isolated from an unidentified starfish in Sanya, China. Antonie van Leeuwenhoek, 2017, 110, 187-194

    18. He C, Li J, Li W, Xue Y, Fang ZM, Fang W, Zhang XC, Wang XT, Xiao YZ.  Structures of PspAG97A α-glucoside hydrolase reveal a novel mechanism for chloride induced activation. Journal of Structural Biology 2016, 196: 426-436

    19. Li W, Fan H, He C, Zhang XC, Wang XT, Yuan J, Fang ZM, Fang W*, Xiao YZ*. PspAG97A: a halophilic α-glucoside hydrolase with novel substrate specificity from glycoside hydrolase family 97. Journal of Microbiology and Biotechnology 2016, 26(11): 1933-1942

    20.  Zhang Y, Tang P, Xu Y, Fang W, Wang X, Fang ZM*, Xiao Y*.Lutimaribacter marinistellae sp. nov., isolated from a starfish. Int J Sys Evo Microb, 2016, 66: 3675-3680, doi: 10.1099/ijsem.0.001251.

    21.  Fang W, Yang Y, Zhang X, Yin Q, Zhang X, Wang X, Fang ZM*, Xiao Y*. Improve ethanol tolerance of β-glucosidase Bgl1A by semi-rational engineering for the hydrolysis of soybean isoflavone glycosides. J Biotechnol, 2016, 227,64-71.

    22.  Chen L, Yi X, Deng F, Fang W, Zhang X, Wang X, Fang ZM*, Xiao Y. A novel ethanol-tolerant laccase, Tvlac, from Trametes versicolor. Biotech Lett, 2016, 38:471–476.

    23. Li W, Xue Y, Li J, Yuan J, Wang X, Fang W, Fang ZM*, Xiao Y. A cold-adapted and glucose-stimulated type II α-glucosidase from a deep-sea bacterium Pseudoalteromonas sp. K8. Biotech Lett, 2016, 38:345–349.